You guys are all wrong! Obviously the best movie scene is when Barney breaks into the "I love you You love me We're a happy family" song!
Mine would be when Darth Vader tells Luke Skywalker he is his father....
LTC. Killgore that's all I should have to save, if you aren't familiar with him...
http://www.youtube.com/watch?v=IcaKOVqI554
Now what was the movie with the two hot babes?